We produce AAV vectors in human embryonic kidney HEK293 cells through three plasmid transfection method, in which one plasmid carried the AAV rep/cap genes, a second the adenovirus helper genes, and a third the gene of interest flanked with two AAV ITRs.

Our production scale of double Cesium Chloride ultracentrifugation purified AAV starts from 1E+12 vg to 1E+15 vg, titer per qPCR results on AAV ITR. Current timeline will be 3-4 weeks and up. Please provide us 1 ug GOI plasmid DNA for the transformation and Maxiprep DNA purification, or provide us your own purified and sterilized plasmid DNA for the triple transfection directly.

(The price listed below is for high yield AAV serotype 2, 5, 8, 9 & PHP.eB)

Alternatively, see here for AAV production in Virovek’s proprietary Sf9 cells under serum-free condition through recombinant baculovirus infection.

Please inquire us for your custom project needs.

HEK293 (Custom) 1.00E+12 Viral Genome $808.00Crude Lysate (In Vitro)
HEK293 (Custom) 5.00E+12 Viral Genome $1,212.00Crude Lysate (In Vitro)
HEK293 (Custom) 1.00E+13 Viral Genome $2,020.00Crude Lysate (In Vitro)

HEK293 (Custom) 1.00E+12 Viral Genome $2,020.00Purified AAV (In Vivo)
HEK293 (Custom) 5.00E+12 Viral Genome $2,525.00Purified AAV (In Vivo)
HEK293 (Custom) 1.00E+13 Viral Genome $3,030.00Purified AAV (In Vivo)

Other titer points up to 2.00E+14 VG On RequestInquire us for details