Understanding Virovek’s Gene Cloning Service

We create constructs for AAV packaging to include toxin genes, IRES or P2A sequences, miRNAs, shRNAs, Cre-dependent, and dual promoter expression cassettes.

STEP ONE
Customer should provide
us with EITHER:


The plasmid containing the target gene


Or the GenBank number/sequence file of the target gene


Destination plasmid for the desired gene

STEP TWO

We design a strategy
to clone the target gene
into the destination
plasmid

STEP THREE

Verifying the resulting construct through
restriction enzyme
digestion and exact sequence analysis

STEP FOUR
You will receive a DETAILED REPORT describing:


The cloning
process


Miniprep of DNA of the plasmid


Agarose gel image showing restriction verification of your plasmid

Specialties and Add-ons

Single-step cloning strategies $395.00/unitExcision of the gene of interest and insertion into the destination plasmid, 1-2 weeks

Complex cloning strategies $250.00/unitFor each; ligation of 3+ pieces of DNA, insertion of fusion proteins or linkers, up to 4 steps