Technologies

Virovek is the leader in adeno-associated virus (AAV) vector design, production, and purification. We have the world's most efficient large-scale AAV manufacturing system through our innovation and patented technologies.

Bac-to-AAV System

rBV dual infection Sf9 Cells

rBV dual infection of Sf9 cells

rAAV production

AAV virus particles

AAV Production Technology

Virovek has developed a patented BAC-to-AAV technology that utilizes the baculovirus expression system to produce AAV vectors in insect cells under serum-free condition. Our AAV vectors have been proven to infect and transduce a myriad of mammalian cell lines in vitro and a broad range of cell types in vivo.
Our AAV technology is recognized as the world's most powerful and efficient AAV production platform. We have the capability to generate over 3e+16vg of AAV vectors with a single production run, which is unmatched by any other AAV production system.

Established a growing list of partnerships in both academia and industry since 2006

BAC-to-AAV technology

Patented BAC-to-AAV technology

Mammalian cell lines in vitro

Infect and transduce a myriad of mammalian cell lines in vitro

AAV production platform

World's most powerful AAV production platform

Efficient AAV production platform

World's most efficient AAV production platform

Three Key Steps to Generating Your Custom-Made AAV Vector

Virovek's AAV shuttle vector

Sub-clone your gene of interest (GOI) into Virovek's AAV shuttle vector

We will design the best cloning strategy, sub-clone your GOI into our AAV shuttle vector, and perform sequence analysis to verify the integrity of your GOI.

Baculo virus

Generate baculovirus containing your GOI

We will then generate bacmid DNA containing your GOI, purify the bacmid DNA, transfect Sf9 insect cells to produce recombinant baculovirus containing your GOI, and then amplify the recombinant baculovirus.

AAV vectors

Produce AAV vectors containing your GOI

Finally, we will double infect Sf9 cells using the amplified recombinant baculovirus containing your GOI and another recombinant baculovirus containing the Rep-Cap genes of your choice to produce recombinant AAV vectors containing your GOI, purify the recombinant AAV vectors, and perform qPCR to determine the viral titer SDS-PAGE to verify the purity of AAV vectors.

Our AAV Production Timeline

The whole AAV production process takes approximately 5-6 weeks to complete (see table below for timeline).

StepsDescriptionEstimated
time required
1Cloning gene of interest (GOI) into Virovek's AAV shuttle plasmid1~2 weeks
2Generation of Bacmid and purification of Bacmid DNA4 days
3Transfection of Sf9 cells to generate baculovirus4 days
4Amplification of baculovirus and titration3 days
5Production of AAV and CsCl purification1 week
6Desalting, filter sterilization, and AAV titration2 days

Virovek's Patented Toxin-Based Cell Ablation Technology

Virovek has developed the world's first and only manufacturing system to produce viral vectors harboring toxin genes. This patented technology also utilizes the same baculovirus expression system to produce AAV vectors in insect cells under serum-free condition.

Toxin genes

The world's first to produce viral vectors harboring toxin genes

AAV vectors harboring toxin genes

Using promoter-specific and/or capsid-specific AAV vectors harboring toxin genes

AAV-toxin vectors in vitro

Cancer cell killing effects of these AAV-toxin vectors in vitro

We have successfully demonstrated the cancer cell killing effects of these AAV-toxin vectors in vitro. In vivo testings of these AAV-toxin vectors in different animal cancer models are now underway.

Learn more

Cell Ablation
Technology

The toxin genes are deactivated during the production process, and only reactivated once the AAV-toxin vectors are produced. Using promoter-specific and/or capsid-specific AAaV vectors harboring toxin genes, this cell ablation technology is able to hone in on and destroy specific subsets of cells within a larger tissue sample or organ.

As a result, this toxin-based cell ablation technology can be used to study the functionality of particular types of cells, as well as for therapeutic purposes by targeting diseased cells.

Products

Virovek is an adeno-associated virus (AAV) production and protein expression services company. With our unique and patented technologies, we provide our customers with:

Toxin-based cell ablation

Technology using AAV vectors as the delivery vehicle

Custom-made AAV

Vector production service from 1E+13vg scale to 3E+16vg scale
results may vary

Safety and Storage

handled in a Biosafety Level 1 (BSL-1) environment

Intermediate Products

sells intermediates from all points in our AAV Production process

Pre-Made AAVs

approximate default titer of 2 x 10^13 vg/mL

 

Services

Virovek is an adeno-associated virus (AAV) production and protein expression services company. With our unique and patented technologies, we provide our customers with:

Gene Cloning Services

Virovek offers gene cloning services for your needs! To get started, the customer will provide us with the plasmid containing the target gene or

Quality Control Assays

Virovek offers a selection of quality control assays that you may select for your AAV products.

Partners and Customers