Introduction to AAV

Adeno-associated viruses (AAVs), from the parvovirus family, are small viruses (22 nm) that are replication-defective...More

Contract Manufacturing

Contract Manufacturing of Custom-Made AAV Vectors

Virovek’s proprietary AAV manufacturing technology has revolutionized the AAV production process. Our AAV production platform can generate over 1E+16vg of AAV vectors with a single production run.

We utilize the baculovirus expression system to produce AAV vectors in insect cells under serum-free condition. Virovek’s recombinant baculoviruses are extremely stable. Even after 5 passages of amplification, all of the single plaques picked at passage 1 show no apparent loss of rep and cap protein expression. The stability of the baculoviruses allows us to obtain large volumes of uniform seed stocks for any large-scale AAV vector production.

Our AAV vectors have been proven to infect and transduce a myriad of mammalian cell lines in vitro and a broad range of cell types in vivo. The efficacy of our AAVs to consistently produce the desired results is evidenced by our growing list of partnerships in both academia and industry.

Please visit our Custom-Made AAVs page for more information.

Materials Needed to Initiate Project

Virovek has a streamlined production process to ensure your custom-made AAV vectors are delivered to you in a timely manner.

We will need you to provide the following:

a) Plasmid DNA containing your gene of interest (GOI), or GenBank number of your GOI
b) Annotated sequence file and/or map of your GOI
c) AAV serotype used for packaging
d) Production scale size

We will design the best cloning strategy, clone your GOI into Virovek's AAV vector production system, and package, produce, and purify the highest quality AAV vectors for you.

Please contact us if you have any questions or if you would like to place an order.